Journal: Experimental and Therapeutic Medicine

Article Title: Role of SARS‑CoV‑2 nucleocapsid protein in affecting immune cells and insights on its molecular mechanisms

doi: 10.3892/etm.2023.12203

Figure Lengend Snippet: Quantification of TNF-α, IL-10, IL-6 and CD163. The concentrations of (A) TNF-α, (B) IL-10, (C) IL-6 and (D) CD163 were determined in the supernatant of cultured Raw264.7 cells treated with the severe acute respiratory syndrome coronavirus 2 N protein. Data are presented as mean ± SD (n=3). * P<0.05 and ** P<0.01 vs. blank control.

Article Snippet: PageRuler TM Prestained protein ladder (cat. no. 26616) was purchased from Thermo Fisher Scientific, Inc. Trypsin-EDTA (0.25%) used to digest Raw264.7 cells for cell passage was purchased from Gibco; Thermo Fisher Scientific Inc. TNF-α Elisa kit (Cat. no. EK0527), IL-6 Elisa Kit (Cat. no. EK0411), IL-10 Elisa Kit (Cat. no. EK0417) and CD163 ELISA kit (Cat. no. EK1146) were purchased from Boster Biological Technology co., Ltd.).

Techniques: Cell Culture, Control

Journal: International Journal of Molecular Sciences

Article Title: Macrophage Activation Markers Predict Liver-Related Complications in Primary Biliary Cholangitis

doi: 10.3390/ijms23179814

Figure Lengend Snippet: Clinical characteristics of patients with primary biliary cholangitis.

Article Snippet: For the non-invasive evaluation of IP, the serum levels of sCD163 were determined using a kit (Cusabio Technology, Houston, TX, USA) [ ], while sMR was measured using an enzyme-linked immunosorbent assay (ELISA) kit (LifeSpan BioSciences, Seattle, WA, USA) [ ].

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Macrophage Activation Markers Predict Liver-Related Complications in Primary Biliary Cholangitis

doi: 10.3390/ijms23179814

Figure Lengend Snippet: Diagnostic accuracy of intestinal permeability markers for symptom development in patients with primary biliary cholangitis.

Article Snippet: For the non-invasive evaluation of IP, the serum levels of sCD163 were determined using a kit (Cusabio Technology, Houston, TX, USA) [ ], while sMR was measured using an enzyme-linked immunosorbent assay (ELISA) kit (LifeSpan BioSciences, Seattle, WA, USA) [ ].

Techniques: Diagnostic Assay, Permeability

Journal: International Journal of Molecular Sciences

Article Title: Macrophage Activation Markers Predict Liver-Related Complications in Primary Biliary Cholangitis

doi: 10.3390/ijms23179814

Figure Lengend Snippet: Receiver operating characteristic curves for soluble mannose receptor and soluble CD163 alone and combined.

Article Snippet: For the non-invasive evaluation of IP, the serum levels of sCD163 were determined using a kit (Cusabio Technology, Houston, TX, USA) [ ], while sMR was measured using an enzyme-linked immunosorbent assay (ELISA) kit (LifeSpan BioSciences, Seattle, WA, USA) [ ].

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Macrophage Activation Markers Predict Liver-Related Complications in Primary Biliary Cholangitis

doi: 10.3390/ijms23179814

Figure Lengend Snippet: Univariate and multivariate analyses of predictive markers for symptom development in patients with primary biliary cholangitis.

Article Snippet: For the non-invasive evaluation of IP, the serum levels of sCD163 were determined using a kit (Cusabio Technology, Houston, TX, USA) [ ], while sMR was measured using an enzyme-linked immunosorbent assay (ELISA) kit (LifeSpan BioSciences, Seattle, WA, USA) [ ].

Techniques: Biomarker Discovery

Journal: International Journal of Molecular Sciences

Article Title: Macrophage Activation Markers Predict Liver-Related Complications in Primary Biliary Cholangitis

doi: 10.3390/ijms23179814

Figure Lengend Snippet: The cumulative incidence of PBC-related complications according to CD163. Patients with PBC having sCD163 of ≥30.1 tended to have more liver complications more frequently than those with sCD163 of <30.1 ( p = 0.0502). PBC: primary biliary cholangitis.

Article Snippet: For the non-invasive evaluation of IP, the serum levels of sCD163 were determined using a kit (Cusabio Technology, Houston, TX, USA) [ ], while sMR was measured using an enzyme-linked immunosorbent assay (ELISA) kit (LifeSpan BioSciences, Seattle, WA, USA) [ ].

Techniques:

Journal: PLoS ONE

Article Title: Association of DPP4 Gene Polymorphisms with Type 2 Diabetes Mellitus in Malaysian Subjects

doi: 10.1371/journal.pone.0154369

Figure Lengend Snippet: Values in the upper represent DPP4 SNPs, while values in the left represent the R 2 value.

Article Snippet: The level of sDPP-IV in the fasting serum from each participant was quantified by the enzyme-linked immunosorbent assay method using the Human CD26 (RayBiotech, Inc., USA) according to the manufacturer’s protocol.

Techniques: